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Image Search Results
Journal: EBioMedicine
Article Title: SARS-CoV-2 entry sites are present in all structural elements of the human glossopharyngeal and vagal nerves: Clinical implications
doi: 10.1016/j.ebiom.2022.103981
Figure Lengend Snippet: Immuno-stained cultured oligodendrocytes and CNS fibroblasts. Cells grown in chamber slides were stained for ACE2, NRP-1 and TMPRSS2 using human immortalized oligodendroglioma cells and human primary brain vascular fibroblast. Chamber slides of each cell line were stained with either ACE2 (a, d) using a rabbit primary antibody and an Alexa-594 (red) conjugated secondary antibody, TMPRSS2 (c, f) using a rabbit primary antibody and an anti-rabbit Alexa-594 (red) conjugated secondary antibody, or an anti-Neuropilin (b, e) pre-conjugated to Alexa-488 antibody. Cell nuclei are stained with DAPI (blue). *All primary antibodies were applied overnight with a 1:100 dilution. *All secondary antibodies were diluted 1:500.
Article Snippet:
Techniques: Staining, Cell Culture